Human genomic DNA content of saliva samples collected with the Oragene® self-collection kit
نویسندگان
چکیده
Estimating the amount of human genomic DNA in oral samples is important because normal oral samples (as compared to blood samples) contain variable amounts of bacterial DNA. We determined that the median bacterial DNA content in a series of 50 Oragene®/saliva samples was 11.8% of the total DNA, suggesting that 88.2% was of human origin. As an independent measure of the human DNA content, the TaqMan® RNase P assay was used. When corrected for efficiency using bacteria-free blood DNA from the same donors, the human DNA content was determined to be 80%. Thus, two complementary methods allow the same conclusion, that the vast majority of DNA in Oragene/saliva samples is of human origin.
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